Structural studies of CDP-tyvelose 2-epimerase
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چکیده
منابع مشابه
Kinetic and structural studies of hydroxyproline 2-epimerase.
Hydroxyproline Z-epimerase, purified to homogeneity from hydroxyproline-induced cells of Pseudomonas pufida, behaved like a single peptide chain of approximately 64,000 molecular weight, based both on ultracentrifugal data and on a study of tryptic peptides and amino acid composition. Several methods indicated a total of 12 reduced cysteines per mole of native enzyme. Of these only six reacted ...
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Tyvelose epimerase catalyzes the last step in the biosynthesis of tyvelose by converting CDP-d-paratose to CDP-d-tyvelose. This unusual 3,6-dideoxyhexose occurs in the O-antigens of some types of Gram-negative bacteria. Here we describe the cloning, protein purification, and high-resolution x-ray crystallographic analysis of tyvelose epimerase from Salmonella typhi complexed with CDP. The enzym...
متن کاملIdentification and sequence of rfbS and rfbE, which determine antigenic specificity of group A and group D salmonellae.
Salmonella group A, group B, and group D strains have paratose, abequose, and tyvelose, respectively, as the immunodominant sugar in their O antigens, which are otherwise identical; only the final steps differ in the biosynthetic pathways of these sugars. The gene rfbJ from a group B strain, encoding abequose synthase, the final and only unique step in the biosynthesis of CDP-abequose, has been...
متن کاملMolecular and Structural Discrimination of Proline Racemase and Hydroxyproline-2-Epimerase from Nosocomial and Bacterial Pathogens
The first eukaryotic proline racemase (PRAC), isolated from the human Trypanosoma cruzi pathogen, is a validated therapeutic target against Chagas' disease. This essential enzyme is implicated in parasite life cycle and infectivity and its ability to trigger host B-cell nonspecific hypergammaglobulinemia contributes to parasite evasion and persistence. Using previously identified PRAC signature...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2002
ISSN: 0108-7673
DOI: 10.1107/s0108767302088700